Simple, Low-Cost Molecular Assays for TR34/L98H Mutations in the cyp51A Gene for Rapid Detection of Triazole-Resistant Aspergillus fumigatus Isolates
نویسندگان
چکیده
منابع مشابه
Simple, low-cost molecular assays for TR34/L98H mutations in the cyp51A gene for rapid detection of triazole-resistant Aspergillus fumigatus isolates.
Simple, low-cost PCR/PCR-restriction fragment length polymorphism (RFLP) assays targeting cyp51A promoter and codon 98 regions were developed for the detection of triazole-resistant Aspergillus fumigatus strains carrying TR34/L98H mutations. The assays were evaluated using 40 itraconazole-susceptible isolates and 35 itraconazole-resistant isolates. The prevalence of TR34/L98H mutations in clini...
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Citation: Postina P, Skladny J, Boch T, Cornely OA, Hamprecht A, Rath P-M, Steinmann J, Bader O, Miethke T, Dietz A, Merker N, Hofmann W-K, Buchheidt D and Spiess B (2018) Comparison of Two Molecular Assays for Detection and Characterization of Aspergillus fumigatus Triazole Resistance and Cyp51A Mutations in Clinical Isolates and Primary Clinical Samples of Immunocompromised Patients. Front. M...
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متن کاملMultiple cyp51A-based mechanisms identified in azole-resistant isolates of Aspergillus fumigatus from China.
Seventy-two A. fumigatus clinical isolates from China were investigated for azole resistance based on mutations of cyp51A. We identified four azole-resistant strains, among which we found three strains highly resistant to itraconazole, two of which exhibit the TR34/L98H/S297T/F495I mutation, while one carries only the TR34/L98H mutation. To our knowledge, the latter has not been found previousl...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 2014
ISSN: 0095-1137
DOI: 10.1128/jcm.00408-14